Blar i forfatter "Bruun, Jack-Ansgar"

Viser treff 1-19 av 19

    • CALCOCO1 acts with VAMP ‐associated proteins to mediate ER ‐phagy 

      Nthiga, Thaddaeus Mutugi; Shrestha, Birendra Kumar; Sjøttem, Eva; Bruun, Jack-Ansgar; Larsen, Kenneth Bowitz; Bhujabal, Zambarlal; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-11)
      The endoplasmic reticulum (ER) plays important roles in protein synthesis and folding, and calcium storage. The volume of the ER and expression of its resident proteins are increased in response to nutrient stress. ER‐phagy, a selective form of autophagy, is involved in the degradation of the excess components of the ER to restore homeostasis. Six ER‐resident proteins have been identified as ER‐phagy ...

    • Cancer-associated fibroblasts from lung tumors maintain their immunosuppressive abilities after high-dose irradiation 

      Gorchs, Laia; Hellevik, Turid; Bruun, Jack-Ansgar; Camilio, Ketil Andre; Al-Saad, Samer; Stuge, Tor Brynjar; Martinez, Inigo Zubiavrre (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-05-12)
      Accumulating evidence supports the notion that high-dose (>5 Gy) radiotherapy (RT) regimens are triggering stronger pro-immunogenic effects than standard low-dose (2 Gy) regimens. However, the effects of RT on certain immunoregulatory elements in tumors remain unexplored. In this study, we have investigated the effects of high-dose radiotherapy (HD-RT) on the immunomodulating functions of cancer-associated ...

    • Changes in the proteome and secretome of rat liver sinusoidal endothelial cells during early primary culture and effects of dexamethasone 

      Li, Ruomei; Bhandari, Sabin; Martinez-Zubiaurre, Inigo; Bruun, Jack-Ansgar; Urbarova, Ilona Halva; Smedsrød, Bård; Simon-Santamaria, Jaione; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-09-02)
      Introduction<p> <p>Liver sinusoidal endothelial cells (LSECs) are specialized fenestrated scavenger endothelial cells involved in the elimination of modified plasma proteins and tissue turnover waste macromolecules from blood. LSECs also participate in liver immune responses. A challenge when studying LSEC biology is the rapid loss of the in vivo phenotype in culture. In this study, we have ...

    • Changes in the secretory profile of NSCLC-associated fibroblasts after ablative radiotherapy: Potential impact on angiogenesis and tumor growth 

      Hellevik, Turid; Pettersen, Ingvild; Berg, Vivian; Bruun, Jack-Ansgar; Bartnes, Kristian; Busund, Lill-Tove; Chalmers, Anthony; Bremnes, Roy M.; Martinez, Inigo Zubiavrre (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-03-05)
      In the context of radiotherapy, collateral effects of ablative ionizing radiation (AIR) on stromal components of tumors remains understudied. In this work, cancer-associated fibroblasts (CAFs) isolated from freshly resected human lung tumors were exposed to AIR (1x18Gy) and analyzed for their release of paracrine factors. Inflammatory mediators and regulators of angiogenesis and tumor growth were ...

    • Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate 

      Cavanagh, Jorunn Pauline; Pain, Maria Charlene Ronessen; Askarian, Fatemeh; Bruun, Jack-Ansgar; Urbarova, Ilona; Wai, Sun Nyunt; Schmidt, Frank; Johannessen, Mona (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-11-22)
      Staphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S. haemolyticus virulence factors is scarce. Bacterial membrane vesicles (MVs) are one mediator of virulence by enabling secretion and long distance delivery ...

    • Comparative exoproteome profiling of an invasive and a commensal Staphylococcus haemolyticus isolate 

      Cavanagh, Jorunn Pauline; Pain, Maria Charlene Ronessen; Askarian, Fatemeh; Bruun, Jack-Ansgar; Urbarova, Ilona; Wai, Sun Nyunt; Schmidt, Frank; Johannessen, Mona (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-22)
      <p><i>Staphylococcus haemolyticus</i> is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of <i>S. haemolyticus</i> are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other <i>S. haemolyticus</i> virulence factors is scarce. Bacterial membrane vesicles (MVs) are one mediator of virulence by enabling secretion and ...

    • Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes 

      Nilsen, Bo Wold; Simon-Santamaria, Jaione; Ørtengren, Ulf Thore; Jensen, Einar; Bruun, Jack-Ansgar; Michelsen, Vibeke Barman; Sørensen, Karen Kristine (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-27)
      Triethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resinbased dental materials. This study investigated the molecular mechanisms of TEGDMA toxicity by identifying its time- and dose-dependent effects on the proteome of human THP-1 monocytes. The effects of different concentrations (0.07– 5 mM) and exposure times (0–72 h) of TEGDMA on cell viability, proliferation, and morphology ...

    • Immunization with lytic polysaccharide monooxygenase CbpD induces protective immunity against Pseudomonas aeruginosa pneumonia 

      Askarian, Fatemeh; Tsai, Chih-Ming; Cordara, Gabriele; Zurich, Raymond H.; Bjånes, Elisabet; Golten, Ole; Sørensen, Henrik Vinther; Kousha, Armin; Meier, Angela; Chikwati, Elvis Mashingaidze; Bruun, Jack-Ansgar; Ludviksen, Judith K; Choudhury, Biswa; Trieu, Desmond; Davis, Stanley; Edvardsen, Per Kristian Thorén; Mollnes, Tom Eirik; Liu, George Y.; Krengel, Ute; Conrad, Douglas J.; Vaaje-Kolstad, Gustav; Nizet, Victor (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-07-17)
      Pseudomonas aeruginosa (PA) CbpD belongs to the lytic polysaccharide monooxygenases (LPMOs), a family of enzymes that cleave chitin or related polysaccharides. Here, we demonstrate a virulence role of CbpD in PA pneumonia linked to impairment of host complement function and opsonophagocytic clearance. Following intratracheal challenge, a PA ΔCbpD mutant was more easily cleared and produced less ...

    • Large-scale secretome analyses unveil the superior immunosuppresive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells 

      Islam, Md Ashraful; Urbarova, Ilona; Bruun, Jack-Ansgar; Martinez, Inigo Zubiavrre (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-20)
      Mesenchymal stromal cells (MSCs), given their regenerative potential, are being investigated as a potential therapeutic tool for cartilage lesions. MSCs express several bioactive molecules which act in a paracrine fashion to modulate the tissue microenvironment. Yet, little is known about the divergence of these signalling molecules in different MSC populations. The present study investigated ...

    • NIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1 

      Shrestha, Birendra Kumar; Rasmussen, Mads Skytte; Abudu, Yakubu Princely; Bruun, Jack-Ansgar; Larsen, Kenneth Bowitz; Alemu, Endalkachew Ashenafi; Sjøttem, Eva; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-12-19)
      Human ATG8 family proteins (ATG8s) are active in all steps of the macroautophagy pathway, and their lipidation is essential for autophagosome formation. Lipidated ATG8s anchored to the outer surface of the phagophore serve as scaffolds for binding of other core autophagy proteins and various effector proteins involved in trafficking or fusion events, whereas those at the inner surface are needed for ...

    • P62/sequestosome-1, autophagy-related gene 8, and autophagy in Drosophila are regulated by nuclear factor erythroid 2-related factor 2(NRF2), independent of transcription factor TFEB 

      Jain, Ashish; Rusten, Tor Erik; Katheder, Nadja Sandra; Elvenes, Julianne; Bruun, Jack-Ansgar; Sjøttem, Eva; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-04-30)
      The selective autophagy receptor p62/sequestosome 1 (SQSTM1) interacts directly with LC3 and is involved in oxidative stress signaling in two ways in mammals. First, p62 is transcriptionally induced upon oxidative stress by the NF-E2-related factor 2 (NRF2) by direct binding to an antioxidant response element in the p62 promoter. Second, p62 accumulation, occurring when autophagy is impaired, leads ...

    • Phosphorylation of Syntaxin 17 by TBK1 Controls Autophagy Initiation 

      Kumar, Suresh; Gu, Yuexi; Abudu, Yakubu Princely; Bruun, Jack-Ansgar; Jain, Ashish; Farzam, Farzin; Mudd, Michal H.; Anonsen, Jan Haug; Rusten, Tor Erik; Kasof, Gary; Ktistakis, Nicholas; Lidke, Keith A; Johansen, Terje; Deretic, Vojo (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-02-28)
      Syntaxin 17 (Stx17) has been implicated in autophagosome-lysosome fusion. Here, we report that Stx17 functions in assembly of protein complexes during autophagy initiation. Stx17 is phosphorylated by TBK1 whereby phospho-Stx17 controls the formation of the ATG13<sup>+</sup>FIP200<sup>+</sup> mammalian pre-autophagosomal structure (mPAS) in response to induction of autophagy. TBK1 phosphorylates Stx17 ...

    • Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding Affinity and Specificity 

      Wirth, Martina; Mouilleron, Stephane; Zhang, Wenxin; Abudu, Yakubu Princely; Jain, Ashish; Olsvik, Hallvard Lauritz; Bruun, Jack-Ansgar; Razi, Minoo; Jefferies, Harold B.J.; Lee, Rebecca; Joshi, Dhira; O'Reilly, Nicola; Johansen, Terje; Tooze, Sharon A (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-04-24)
      <p>Autophagy is a highly conserved degradative pathway, essential for cellular homeostasis and implicated in diseases including cancer and neurodegeneration. Autophagy-related 8 (ATG8) proteins play a central role in autophagosome formation and selective delivery of cytoplasmic cargo to lysosomes by recruiting autophagy adaptors and receptors. The LC3-interacting region (LIR) docking site (LDS) of ...

    • The Presumed Polyomavirus Viroporin VP4 of Simian Virus 40 or Human BK Polyomavirus Is Not Required for Viral Progeny Release 

      Henriksen, Stian; Hansen, Terkel; Bruun, Jack-Ansgar; Rinaldo, Christine Hanssen (Journal article; Tidsskriftartikkel; Peer reviewed, 2016-09-14)
      The minor capsid protein of human BK polyomavirus (BKPyV), VP2, and its N-terminally truncated form, VP3, are both important for viral entry. The closely related simian virus 40 (SV40) reportedly produces an additional truncated form of VP2/3, denoted VP4, apparently functioning as a viroporin promoting progeny release. The VP4 open reading frame is conserved in some polyomaviruses, including BKPyV. ...

    • Profiling the Atlantic Salmon IgM+ B Cell Surface Proteome: Novel Information on Teleost Fish B Cell Protein Repertoire and Identification of Potential B Cell Markers 

      Penaranda, Ma Michelle Demogina; Jensen, Ingvill; Tollersrud, Linn G.; Bruun, Jack-Ansgar; Jørgensen, Jorunn B. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-01-29)
      Fish immunology research is at a pivotal point with the increasing availability of functional immunoassays and major advances in <i>omics</i> approaches. However, studies on fish B cells and their distinct subsets remain a challenge due to the limited availability of differentially expressed surface markers. To address this constraint, cell surface proteome of Atlantic salmon IgM<sup>+</sup> B cells ...

    • Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy 

      Nthiga, Thaddaeus Mutugi; Shrestha, Birendra Kumar; Bruun, Jack-Ansgar; Larsen, Kenneth Bowitz; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-04-19)
      The Golgi complex is essential for the processing, sorting, and trafficking of newly synthesized proteins and lipids. Golgi turnover is regulated to meet different cellular physiological demands. The role of autophagy in the turnover of Golgi, however, has not been clarified. Here we show that CALCOCO1 binds the Golgi-resident palmitoyltransferase ZDHHC17 to facilitate Golgi degradation by autophagy ...

    • Regulation of Transcriptional Activity of Merkel Cell Polyomavirus Large T-Antigen by PKA-Mediated Phosphorylation 

      Falquet, Mar; Prezioso, Carla; Ludvigsen, Maria A.; Bruun, Jack-Ansgar; Passerini, Sara; Sveinbjørnsson, Baldur; Pietropaolo, Valeria; Moens, Ugo (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-01-03)
      Merkel cell polyomavirus (MCPyV) is the major cause of Merkel cell carcinoma (MCC), an aggressive skin cancer. MCPyV large T-antigen (LTag) and small T-antigen (sTag) are the main oncoproteins involved in MCPyV-induced MCC. A hallmark of MCPyV-positive MCC cells is the expression of a C-terminal truncated LTag. Protein kinase A (PKA) plays a fundamental role in a variety of biological processes, ...

    • Starvation induces rapid degradation of selective autophagy receptors by endosomal microautophagy 

      Mejlvang, Jakob; Olsvik, Hallvard Lauritz; Svenning, Steingrim; Bruun, Jack-Ansgar; Abudu, Yakubu Princely; Larsen, Kenneth Bowitz; Brech, Andreas; Hansen, Tom Egil; Brenne, Hanne Britt; Hansen, Terkel; Stenmark, Harald Alfred; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-07-17)
      It is not clear to what extent starvation-induced autophagy affects the proteome on a global scale and whether it is selective. In this study, we report based on quantitative proteomics that cells during the first 4 h of acute starvation elicit lysosomal degradation of up to 2–3% of the proteome. The most significant changes are caused by an immediate autophagic response elicited by shortage of amino ...

    • The third helix of the homeodomain of paired class homeodomain proteins acts as a recognition helix both for DNA and protein interactions 

      Bruun, Jack-Ansgar; Thomassen, Ernst I.s.; Kristiansen, Kurt; Tylden, Garth Daryl; Holm, Turid; Mikkola, Ingvild; Bjørkøy, Geir; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2005)
      The transcription factor Pax6 is essential for the development of the eyes and the central nervous system of vertebrates and invertebrates. Pax6 contains two DNA-binding domains; an N-terminal paired domain and a centrally located homeodomain. We have previously shown that the vertebrate paired-less isoform of Pax6 (Pax6ΔPD), and several other homeodomain proteins, interact with the full-length ...